This is the Scientific Surgery Archive, which contains all randomized clinical trials in surgery that have been identified by searching the top 50 English language medical journal issues since January 1998. Compiled by Jonothan J. Earnshaw, former Editor-in-Chief, BJS
Response of visceral peritoneum to abdominal surgery. BJS 2001; 88: 148-151.
Published: 6th December 2002
Authors: M.‐L. Ivarsson, P. Falk, L. Holmdahl
Background
Postoperative adhesion formation has been associated with a reduced capacity to degrade fibrin within the peritoneal cavity. Peritoneal fibrinolytic capacity has been shown to decrease during the course of a surgical operation. The aim of this study was to investigate whether tissue‐type plasminogen activator (tPA), a key fibrinolytic enzyme, is released into the peritoneal cavity during operation.
Method
Fluid released from the serosal surface of the small bowel was collected in a plastic bag from 16 patients undergoing surgery. Intraoperative blood samples were also taken from seven patients. Concentrations of the fibrinolytic components tPA and urokinase plasminogen activator (uPA), tPA activity and plasminogen activator inhibitor type 1 (PAI‐1) concentration were measured by enzyme‐linked immunoabsorbent assay.
Results
Intraoperative tPA concentrations were significantly raised in the peritoneal fluid collected compared with peripheral blood levels (P = 0·008). This resulted in a significantly higher tPA activity in the fluid compared with blood (P = 0·001). However, neither uPA (P = 0·29) nor PAI‐1 (P = 0·84) concentrations differed significantly in fluid compared with blood.
Conclusion
These data suggest that tPA is rapidly released by the visceral peritoneum during abdominal surgery. The different concentrations in peripheral blood and peritoneum suggest that tPA is released from the peritoneum by an active process, and does not solely derive from leakage of plasma.
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